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Central research question of my study

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What am I doing?

Gene therapy via AAV based strategy

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Generation of hiPSC derived NPCs

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Behavioral assessment for motor function

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Technical skills 

Cell culture

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Culturing HEK293T, Primary cortical neurons, hiPSC derived Neural Progenitor Cells (NPCs) and Spinal cord Progenitors (SCPs)

Molecular Biology

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Cut and paste and PCR based cloning, PEI and Lipofectamine based transfection, AAV-preparation, Lentivirus preparation, CRISPR-Knock in 

Animal Handling

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AAV-based Anterograde injections in motor cortex, Retrograde labelling using AAV, Thoracic crush injury, Neural progenitor transplantation

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